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KMID : 0620920150470020003
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Experimental & Molecular Medicine
2015 Volume.47 No. 2 p.3 ~ p.3
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MiR-34a, miR-21 and miR-23a as potential biomarkers for coronary artery disease: a pilot microarray study and confirmation in a 32 patient cohort
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Hui Han
Guangjin Qu
Chenghua Han
Yuhong Wang
Tingting Sun
Fengqing Li
Junxiao Wang
Shanshun Luo
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Abstract
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The aim of this study was to investigate the expression of circulating microRNAs (miRNAs) in apolipoprotein E (apoE) knockout mice (apoE?/?) and to validate the role of these miRNAs in human coronary artery disease (CAD). Pooled plasma from 10 apoE?/? mice and 10 healthy C57BL/6 (B6) mice was used to perform the microarray analysis. The results showed that miR-34a, miR-21, miR-23a, miR-30a and miR-106b were differentially expressed in apoE?/? mice, and these expression changes were confirmed by real-time quantitative reverse-transcription PCR. Then, miR-34a, miR-21, miR-23a, miR-30a and miR-106b were detected in the plasma of 32 patients with CAD and of 20 healthy controls. Only miR-34a, miR-21 and miR-23a were significantly differentially expressed in the plasma of CAD patients (all P<0.01). In conclusion, miR-34a, miR-21 and miR-23a were elevated in CAD patients, which means that these miRNAs might serve as biomarkers of CAD development and progression.
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KEYWORD
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